CHARACTERIZATION OF AN HFR STRAIN OF SHIGELLA FLEXNERI

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Abstract
Schneider, Herman (Walter Reed Army Institute of Research, Washington, D.C.), and Stanley Falkow . Characterization of an Hfr strain of Shigella flexneri . J. Bacteriol. 88: 682–689. 1964.—A Hfr Shigella flexneri , strain 69, was obtained by terminal marker selection in a cross between Hfr Escherichia coli and S. flexneri . The chromosome of this Hfr Shigella bears gross homology to the E. coli chromosome: it can conjugate with both Shigella and E. coli; its order of gene transmission is the same as E. coli; and interrupted matings show that distance between gene loci is the same as for E. coli . The kinetics of transfer of the pro + , thr + + leu + , and arg + loci by Hfr S. flexneri differ from Hfr E. coli , and may indicate that function of the sex factor, F, derived from E. coli , is modified when integrated into the Shigella chromosome.