Hormonal Effects on the Biosynthesis of Sulfated Glycoprotein in a Microsomal Fraction of the Endometrium of Rabbit Uterus1
- 1 February 1978
- journal article
- research article
- Published by Oxford University Press (OUP) in The Journal of Biochemistry
- Vol. 83 (2) , 537-542
- https://doi.org/10.1093/oxfordjournals.jbchem.a131941
Abstract
A crude microsomal fraction (M-Fr) was separated from the endometrial scrapings of uteri of ovariectomized rabbits with or without hormonal treatment. The effects of estrogen and progesterone on the incorporation into M-Fr of L-[U-14C]-fucose and N-acetyl-D-[6-3H]-glucosamine from their nucleotides were investigated. Estrogen increased the incorporation of these sugars, whereas progesterone suppressed this effect. The results of fractionation on a DEAE-Sephadex A-25 (C1- form) column of the isotope-labelled complex saccharide mixtures, obtained by pronase digestion of the incubation mixtures, indicated that biosynthesis of sulfated glycoprotein was most sensitive to the hormones among the complex saccharides in M-Fr. Thus, a hormonal effects on the biosynthesis of sulfated glycoprotein in the endometrium of ovariectomized rabbit has been unambiguously confirmed at the microsomal level.This publication has 2 references indexed in Scilit:
- Effect of eatradiol and progesterone on UDPgalactose pyrophosphatase activity in the endometrium of ovariectomized ratsBiochimica et Biophysica Acta (BBA) - General Subjects, 1976
- Biosynthesis of Sulfated Glycoprotein in the Endometrium of Rabbit Uterus1The Journal of Biochemistry, 1976