Enzymatic Modification of Amylase Inhibitor Produced byStreptomycessp. No. 280

Abstract
At least 4 kind s of amylase inhibitors are found in culture of Streptomyes sp. No. 280. A large amount of amylase inhibitors were produced by Streptomyces sp. No. 280 when cultivated on 3% oatmeal medium and MW of the inhibitors were transformed to smaller molecules during the cultivation time. The transformation of the amylase inhibitor resulted from degradation of its carbohydrate moiety by .alpha.-amylase in the culture broth. The amylase inhibitor was hydrolyzed partially by the action of taka-amylase A or hog pancreatic .alpha.-amylase. With hydrolyzation of amylase inhibitor by .alpha.-amylase, neutral sugar (mainly maltose) were liberated from the amylase inhibitor and a modified inhibitor was newly formed, but anylase inhibitory activity glucoamylase was not changed. The inhibitory activity against [rabbit] muscle phosphorylase was almost completely lost.

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