Reversed-Phase Chromatographic Separation of Benzo[A]Pyrene Metabolites with β-Cyclodextrin as a Mobile Phase Modifier

Publisher Website
Google Scholar
Abstract
β-Cyclodextrin (β-CD) was investigated as a mobile phase modifier for the separation of fourteen metabolites of benzo[a]pyrene. A wide range of β-CD concentrations was employed, and the chromatographic properties of four different classes of metabolites were compared in methanol-water, acetonitrile-water, and methanol-acetonitrile-water mobile phases with and without β-CD. The chromatographic bands for all the metabolites sharpened with β-CD in the mobile phase. The resolution for four classes of metabolites improved with β-CD in the binary and ternary mobile phases. The monohydroxyl-benzo[a]pyrenes used in this study were structurally similar and difficult to separate with methanol-water. However, with β-CD in the mobile phase, overall improvement in the separation of the monohydroxyl-benzo[a]pyrenes was achieved. Substantial improvement was obtained for the separation of 6-hydroxyl-benzo[a]pyrene from 12-hydroxyl-benzo[a]pyrene with β-CD in methanol-water and for 2-hydroxyl-benzo[a]pyrene from 9-hydroxyl-benzo[a]pyrene with β-CD in the acetonitrile-water.

This publication has 19 references indexed in Scilit: