Isolation of the antigen-active components from leptospiral serovar-specific lipopolysaccharide antigen by mild acid hydrolysis.
- 1 January 1982
- journal article
- research article
- Published by Japanese Society of Veterinary Science in The Japanese Journal of Veterinary Science
- Vol. 44 (3) , 473-478
- https://doi.org/10.1292/jvms1939.44.473
Abstract
The serovar-specific main antigen (TM antigen) from Leptospira interrogans-kremastos strain Kyoto was mildly hydrolyzed with 2 N formic acid or 0.5 N sulfuric acid. Both treatments yielded an immunologically active component which was able to pass through a Diaflo membrane filter. The compound obtained by formic acid hydrolysis was shown to be more homogeneous by molecular sieve gel chromatography than that obtained by sulfuric acid hydrolysis. The MW of the former compound was estimated to be .apprx. 3000 daltons. The compound consisted mainly of carbohyhdrates, and it lost almost all of its fatty acids and proteins. The substance lost its immunoprecipitable activity but showed serovar-specific inhibitory potency in a radioimmuoassay system.This publication has 1 reference indexed in Scilit:
- Inhibition of leptospiral agglutination by the type-specific main antigens of leptospirasInfection and Immunity, 1977