Acetoin Production in the Identification of Isolates as Members of Staphylococcus intermedius Hajek

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Abstract
A total of 50 staphylococcal strains of animals origin were identified as members of S. intermedius Hajek on the basis that they possessed all of the characteristics originally reported by Hajek for S. intermedius except one, acetoin production. The Barritt method failed to demonstrate acetoin production by these isolates, whereas in the case of a micromethod in which pyruvate was used as substrate, acetoin production was detected. The discrepancy observed between the results of the 2 methods and the taxonomic implications of the findings are discussed.

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