Naringin Levels in Citrus Tissues

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Abstract
The preparation of a tritiated radiotracer that was used in the radioimmunoassay of naringin (naringenin-7-O-.alpha.-rhamnosyl-(1-2)-.beta.-D-glucopyranoside) [A bitter flavor component of Citrus paradisi] and which was synthesized by reduction of the carbonyl group of the flavanone is reported. The resulting assay has a detection limit of 0.5 pmol/0.1 ml, is specific for the 7-neohesperidoside substitution on flavanones, and can measure naringin in crude extracts of plant tissues. This radioimmunoassay is compared with 3 other naringin immunoassays which use antibodies raised against 2 different haptens and different tracers labeled with 125I or 3H. The applicability of the methods to the quantification of naringin and other flavanone neohesperidosides in citrus tissue is discussed.