Superoxol(catalase)test for identification of Neisseria gonorrhoeae

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Abstract
The Superoxol test (catalase test using 30% H2O2) was used to differentiate N. gonorrhoeae from other Neisseria spp. A positive test was defined as immediate, brisk bubbling upon dropping 30% H2O2 onto a bacterial colony. Of the gonococci, 100% were Superoxol positive. Only 1% of Superoxol-positive isolates on Thayer-Martin agar were organisms other than gonococci (99% specificity). The test was more reliable than the co-agglutination test. Individual strains of a wide variety of Neisseria and Branhamella spp. were Superoxol positive. They could usually be differentiated from N. gonorrhoeae by their poor growth on selective media, colonial morphology on nonselective media and simple biochemical tests. The Superoxol test is an excellent screening test for N. gonorrhoeae. A positive result on a clinical isolate [human] growing on Thayer-Martin agar is strongly suggestive of the presence of gonococci.

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