DEMONSTRATION OF ESTROGEN-RECEPTORS BY MONOCLONAL-ANTIBODY IN FORMALIN-FIXED BREAST-TUMORS
- 1 March 1988
- journal article
- research article
- Vol. 58 (3) , 346-353
Abstract
An immunohistochemical technique using a commercially available monoclonal antibody to the estrogen receptor in formalin-fixed breast tumors is described. The author''s technique is compared to three other recently published techniques in 27 case studies. The authors'' method using pronase enzyme pretreatment and alkaline phosphatase as the third antibody yielded the best results. Comparison with the standard dextran-coated charcoal cytosolic assay results from the cases selected yielded a 100% sensitivity and 89% specificity for the authors'' technique. The advantages of the immunohistochemical method over the biochemical assay are discussed and clinical implications are suggested. A step-to-step procedure for the author''s technique follows the text.This publication has 4 references indexed in Scilit:
- Detection of Estrogen Receptors with Monoclonal Antibodies in Routinely Processed Formalin-Fixed Paraffin Sections of Breast Carcinoma: Use of DNase Pretreatment to Enhance Sensitivity of the ReactionAmerican Journal of Clinical Pathology, 1987
- COMPARISON OF IMMUNOCYTOCHEMICAL AND STEROID-BINDING ASSAYS FOR ESTROGEN-RECEPTOR IN HUMAN-BREAST TUMORS1985
- Monoclonal antibodies to human estrogen receptor.Proceedings of the National Academy of Sciences, 1980
- Estrogen Receptor Assay of Carcinomas of the Breast by a Simplified Dextran–Charcoal MethodAmerican Journal of Clinical Pathology, 1978