Isolation and preliminary characterization of the GAL4 gene, a positive regulator of transcription in yeast.
- 1 November 1982
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 79 (22) , 6827-6831
- https://doi.org/10.1073/pnas.79.22.6827
Abstract
The GAL4 locus encodes a positive regulator of the inducible galactose and melibiose genes of yeast. Using the yeast plasmid vector YEp13, GAL4 was cloned by complementation of a gal4 mutation. Restriction endonuclease mapping of subclone DNA has delimited the region sufficient for complementation to a 3.2-kilobase segment of DNA. The GAL4 mRNA is 2.8 kilobases long, sufficient to encode a protein as large as 105,000 daltons. The concentration of the GAL4 transcript is about 0.1 per cell, and is almost identical in galactose-induced and non-induced cells. This result is consistent with a previously proposed model in which the activity of the GAL4 protein and not the transcription of the GAL4 gene is modulated by galactose induction.This publication has 23 references indexed in Scilit:
- Isolation of galactose-inducible DNA sequences from Saccharomyces cerevisiae by differential plaque filter hybridizationCell, 1979
- Evidence for transcriptional regulation of orotidine-5'-phosphate decarboxylase in yeast by hybridization of mRNA to the yeast structural gene cloned in Escherichia coli.Proceedings of the National Academy of Sciences, 1979
- Constitutive synthesis of the GAL4 protein, a galactose pathway regulator in saccharomyces cerevisiaeCell, 1979
- Transformation of yeast by a replicating hybrid plasmidNature, 1978
- Transformation of yeast.Proceedings of the National Academy of Sciences, 1978
- Method for detection of specific RNAs in agarose gels by transfer to diazobenzyloxymethyl-paper and hybridization with DNA probes.Proceedings of the National Academy of Sciences, 1977
- Number and distribution of polyadenylated RNA sequences in yeastCell, 1977
- Interaction of super-repressible and dominant constitutive mutations for the synthesis of galactose pathway enzymes in Saccharomyces cerevisiaeMolecular Genetics and Genomics, 1977
- Efficient transcription of RNA into DNA by avian sarcoma virus polymeraseBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1976
- Nucleotide sequence of the rightward operator of phage lambda.Proceedings of the National Academy of Sciences, 1975