ENZYME-LINKED IMMUNOABSORBENT ASSAY OF FACTOR-VIII-RELATED ANTIGEN - INTEREST IN STUDY OF VONWILLEBRANDS DISEASE

  • 1 January 1977
    • journal article
    • research article
    • Vol. 25, 18-24
Abstract
Factor VIII-related antigen [human] was measured by ELISA [enzyme linked immunoabsorbent assay]. Factor VIII R: Ag was first captured between rabbit antibodies to human factor VIII R: Ag adsorbed in polystyrene tubes, and conjugate (peroxidase-labeled rabbit antibodies). A chromogenic enzyme substrate was then added and absorbence measurement allowed subsequent assay. This method was sensitive, reproducible and specific. The comparison between the results obtained by this enzyme immuno-assay and the electro-immunodiffusion assay (EIA) was interesting for the study of quantitative and qualitative variations of factor VIII R: Ag in von Willebrand''s disease. Two observations of von Willebrand variants were described in detail. VIII R: Ag plasma level of the 1st one was normal using EIA but decreased using ELISA, its electrophoretic mobility was increased. In the 2nd case typical anomalies of von Willebrand were found except the VIII R: Ag level which was normal by the 2 methods.