BIOLOGICAL OXIDATION OF NAPHTHALENE

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Abstract
Conditions have been established for the oxidation of naphthalene by a pseudomonad based on the yields of ether extractable substances. Vigorous aeration yielded approximately three times the product obtained from unaerated controls. Oxidations with media adjusted initially to pH 8 gave higher yields than those adjusted to lower pH levels. Calcium and copper appeared to be necessary for maximum yields. Under the conditions described, the non-naphthalenic ether extractable substances amounted to as much as 29% of the original 1% naphthalene. Purification and analysis of the crude extract showed that it consisted largely of salicylic acid. Some properties of the remaining material are described.
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