A DEVICE FOR GROWING BACTERIAL POPULATIONS UNDER STEADY STATE CONDITIONS

Abstract
A device called the breeder is described in which the turbidity of an exponentially growing population of bacteria is maintained at a preset value by controlling the rate of influx of fresh nutrient solution into the culture while the total culture volume reamins constant. The rate of influx of the nutrient solution is controlled by a photocell which responds to the turbidity of the culture and the culture volume is held constant by means of an overflow siphon from the growth tube. A technique is described for overcoming the difficulties of bacterial growth on the walls of the growth tube.

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