A DEVICE FOR GROWING BACTERIAL POPULATIONS UNDER STEADY STATE CONDITIONS
Open Access
- 20 November 1955
- journal article
- research article
- Published by Rockefeller University Press in The Journal of general physiology
- Vol. 39 (2) , 261-266
- https://doi.org/10.1085/jgp.39.2.261
Abstract
A device called the breeder is described in which the turbidity of an exponentially growing population of bacteria is maintained at a preset value by controlling the rate of influx of fresh nutrient solution into the culture while the total culture volume reamins constant. The rate of influx of the nutrient solution is controlled by a photocell which responds to the turbidity of the culture and the culture volume is held constant by means of an overflow siphon from the growth tube. A technique is described for overcoming the difficulties of bacterial growth on the walls of the growth tube.Keywords
This publication has 5 references indexed in Scilit:
- MUTATION RATES OF BACTERIA IN STEADY STATE POPULATIONSThe Journal of general physiology, 1955
- APPARATUS FOR MAINTAINING BACTERIAL CULTURES IN THE STEADY STATEThe Journal of general physiology, 1954
- AUTOMATIC RECORDING OF THE GROWTH RATES OF CONTINUOUSLY CULTURED MICROORGANISMSThe Journal of general physiology, 1953
- Microbial SelectionScience, 1952
- EXPERIMENTS ON SPONTANEOUS AND CHEMICALLY INDUCED MUTATIONS OF BACTERIA GROWING IN THE CHEMOSTATCold Spring Harbor Symposia on Quantitative Biology, 1951