African Trypanosomes: Cultivation of Animal-Infective Trypanosoma brucei in Vitro
- 27 May 1977
- journal article
- research article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 196 (4293) , 992-994
- https://doi.org/10.1126/science.558652
Abstract
Trypanosoma brucei grew in the presence of bovine fibroblast-like cells in Hepes-buffered RPMI 1640 medium with 20 percent fetal bovine serum for more than 220 days at 37 degrees C. The organisms grown in this system were infective to mammalian hosts, retained the morphological and biochemical characteristics of long slender bloodstream forms, and displayed variant-antigen on their surfaces.This publication has 13 references indexed in Scilit:
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