Abstract
When leaf cells of white pine, Pinus strobus L., were plasmolyzed in appropriate concentrations of glucose (0.85-0.95 M) they could be fixed in a mixture of formalin, acetic acid and alcohol, embedded in polyethylene glycol, and measurements of the degree of plasmolysis made without development of artifacts. Fresh material was prepared by freehand sectioning only with difficulty and did not always give reliable results, whereas embedding and microtome sectioning provided satisfactory material for determining the osmotic characteristics of pine foliage tissue.

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