Isolation and characterization of two plasmids from Bifidobacterium longum
Open Access
- 1 July 1997
- journal article
- research article
- Published by Oxford University Press (OUP) in Letters in Applied Microbiology
- Vol. 25 (1) , 5-7
- https://doi.org/10.1046/j.1472-765x.1997.00059.x
Abstract
In order to develop a cloning vector system which can be used in Bifidobacterium sp., we screened about 100 bifidobacteria from the faeces of adults and children. Among them, only one strain, identified as B. longum KJ, was shown to contain extrachromosomal DNAs. Bifidobacterium longum KJ showed multiple plasmid DNA bands which were resolved to be multimers of two plasmids designated pKJ36 and pKJ50. These plasmids were cloned into the Escherichia coli vector pUC19 as pMS36 and pMS50, respectively, and restriction‐mapped.Keywords
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