APPLICATION OF THE BARR AND STROUD GN5 DENSITOMETER FOR DESCRIPTION OF THE CHROMATIN STRUCTURE

  • 1 January 1980
    • journal article
    • research article
    • Vol. 83  (4) , 317-324
Abstract
Nuclei isolated from rat liver can be separated on non-parenchymal diploid, parenchymal diploid and tetraploid nuclei. These nuclei are studied in their chromatin structure using a Barr and Stroud GN5 integrating densitometer. The measuring instrument has the capability to read an integrated absorbance and, as a 2nd value, the area of an absorbing object at different levels of extinction. The projection area of Feulgen-stained nuclei were measured at 557 nm at different extinction levels. Apparently, the chromatin structure of parenchymal diploid and tetraploid nuclei is similar; the chromatin structure of non-parenchymal nuclei is more condensed. This method can be applied for an objective description of the pattern of chromatin density.

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