MEASUREMENT OF TRITIATED-THYMIDINE LABELING INDEX BY INCUBATION INVITRO OF SURGICALLY REMOVED CERVICAL-CANCER

  • 1 January 1977
    • journal article
    • research article
    • Vol. 68  (3) , 307-313
Abstract
Autoradiographic study was made in 24 cases of uterine cervical tumor, including 22 cases of squamous cell carcinoma, using a technique of in vitro labeling of surgical specimens with 3H thymidine (3H-TdR) under hyperbaric O2. Labeling index of the cervical cancer ranged from 8.7-30.4% and was well correlated with the histological subtypes (spinal, transitional and basal cell types). The upper limit of the growth fraction was also estimated from the general relationship between phase lengths. The necessity of sufficient O2 tenstion for S phase cells to effect synthesis of DNA was stressed.