Synthesis and Lectin Recognition of Polystyrene Core−Glycopolymer Corona Nanospheres

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Abstract
Polymeric nanospheres with a polystyrene core and a glucosyloxyethyl methacrylate (GEMA) oligomer corona were synthesized by the free radical coplymerization of styrene (M1) plus a GEMA macromonomer (M2) at various molar ratios (M1/M2 = 50−150) in the presence of AIBN (1 mol % to the total monomer) in an ethanol/water (3/2, v/v) solvent. The size of the nanospheres was controlled from 300 to 620 nm by altering the monomer ratio. The size distributions were significantly narrow. The amount of glucose conjugated per unit surface area of the nanosphere, which was analyzed by the anthron−sulfuric acid method, was 1.01−2.28 μg cm-1, which increased with an increase in size. The transmittance of a solution of dispersed nanospheres (the corresponding glucose concentration was 73 μM) increased by the addition of the glucose-binding protein concanavalin A (Con A) (1−50 μM), indicating that the nanospheres were being precipitated by the cross-linking of ConA. An enzyme-linked lectin assay (ELLA) revealed that Con A bound to the glucose on the nanospheres 250−700-fold more than to monomeric glucose. The binding activity to the nanospheres was less than that to a GEMA oligomer, and decreased with an increase in the amount of GEMA oligomer grafted onto the nanosphere, possibly because of steric hindrance of the lectin binding to the glucose on the nanospheres. The polystyrene core−glycopolymer corona nanosphere is a useful material for studying sugar−biomolecule recognition.

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