RNA duplex unwinding activity of alfalfa mosaic virus RNA-dependent RNA polymerase
- 11 September 1995
- journal article
- Published by Wiley in FEBS Letters
- Vol. 371 (3) , 219-222
- https://doi.org/10.1016/0014-5793(95)00875-a
Abstract
An RNA-dependent RNA polymerase (RdRp) purified from alfalfa mosaic virus-infected tobacco is capable of synthesizing in vitro full-size RNAs of minus and plus polarities. However, the enzyme is not able to perform a complete replication cycle in vitro. The products were found to be completely base-paired to their templates. The enzyme was able to use double-stranded RNA as a template for RNA synthesis if it could initiate from a single-stranded promoter. The inability (of most) of our enzyme preparations to create a single-stranded initiation site could explain why they could not perform a complete replication cycle in vitro. This is the first report on duplex RNA unwinding activities by a plant viral RdRpKeywords
This publication has 14 references indexed in Scilit:
- PLANT VIRAL RNA SYNTHESIS IN CELL-FREE SYSTEMSAnnual Review of Phytopathology, 1994
- Complete replication of a satellite RNA in vitro by a purified RNA-dependent RNA polymeraseJournal of General Virology, 1992
- Replication of cucumber mosaic virus satellite RNA in vitro by an RNA‐dependent RNA polymerase from virus‐infected tobaccoFEBS Letters, 1991
- Analysis of the protein composition of alfalfa mosaic virus RNA-dependent RNA polymeraseVirology, 1991
- Role of alfalfa mosaic virus coat protein gene in symptom formationVirology, 1991
- cis-Acting elements involved in replication of alfalfa mosaic Virus RNAs in vitroVirology, 1990
- Temperature-gradient gel electrophoresisBiophysical Chemistry, 1987
- Coat protein blocks the in vitro transcription of the virion RNAs of alfalfa mosaic virusFEBS Letters, 1986
- Preferential binding of 3'-terminal fragments of alfalfa mosaic virus RNA 4 to virionsBiochemistry, 1980
- Coat protein binds to the 3'-terminal part of RNA 4 of alfalfa mosaic virusBiochemistry, 1978