Liquid-chromatographic measurement of urinary monosaccharides.
Open Access
- 1 August 1980
- journal article
- research article
- Published by Oxford University Press (OUP) in Clinical Chemistry
- Vol. 26 (9) , 1348-1350
- https://doi.org/10.1093/clinchem/26.9.1348
Abstract
We describe "high-performance" liquid chromatography of urinary monosaccharides on a macroporous anion-exchange resin (Hitachi 3013N), with acetonitrile/water as eluent. The separated xylose, arabinose, D-fructose, mannose, galactose, and D-glucose were detected by means of a post-column reduction reaction of tetrazolium blue. Absolute limits of detection were about 10 ng of monosaccharide. Lactose can also be measured.This publication has 3 references indexed in Scilit:
- Analysis of mono- and disaccharides by high-performance liquid chromatography of the benzyloxime-perbenzoyl derivativesJournal of Chromatography A, 1978
- Sensitive determination of derivatized carbohydrates by high-performance liquid chromatographyJournal of Chromatography A, 1977
- An Automated Procedure for Separation of Monosaccharides on Ion Exchange Resins.Acta Chemica Scandinavica, 1965