THE ESCHERICHIA-COLI RAS-LIKE PROTEIN (ERA) HAS GTPASE ACTIVITY AND IS ESSENTIAL FOR CELL-GROWTH
- 1 June 1988
- journal article
- research article
- Vol. 2 (6) , 539-544
Abstract
The era gene of Escherichia coli encodes a protein (Era) which is similar to the eucaryotic RAS family of proteins. We report here that purified Era possesses both GTP-binding and GTPase activities. Era is also shown to be loosely associated with the inner membrane of E. coli. Overproduction of Era to 5% of the total cellular protein does not apparently alter either cell growth or cAMP levels. Disruption of the era gene by insertional inactivation is shown to be lethal by construction of a conditional lethal era mutant strain.This publication has 3 references indexed in Scilit:
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- A 37 × 103 molecular weight plasmid-encoded protein is required for replication and copy number control in the plasmid pSC101 and its temperature-sensitive derivative pHS1Journal of Molecular Biology, 1984
- The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primersGene, 1982