An Atypical Strain of Pseudomonas aeruginosa

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Abstract
A strain of P. aeruginosa, first isolated from urine, could be cultivated in a phosphate-buffered soln. containing creatinine as the sole source of C and N. The strain possesses a specific creatinine-decompos-ing enzyme, tentatively named creatinase, which is not lost after repeated transfers through nutrient broth without creatinine. The creatinase acts on glycocyamidine, but this compound is inadequate for serving as the sole source of C and N for the growth of the atypical strain. Sarcosine, a hydrolytic product of creatinine and creatine, represents a good culture medium for the strain under study and 5 other strains of P. aeruginosa and P. fluorescens tested. Glycine, a hydrolytic product of glycocyamidine, is a poor nutrient for all strains. Cultures of the strain were preserved on nutrient agar aerobically or on 2% creatinine phosphate agar under oil for 45 and 60 days, respectively. Cultures kept aerobically on creatinine phosphate agar or on 2% creatinine nutrient agar lost their viability, but not their creatinase activity within 8-10 days.

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