Human milk lactoferrin binds two DNA molecules with different affinities
- 28 May 1999
- journal article
- Published by Wiley in FEBS Letters
- Vol. 451 (3) , 235-237
- https://doi.org/10.1016/s0014-5793(99)00579-7
Abstract
Evidence is presented that lactoferrin (LF), an Fe3+-binding glycoprotein, possesses two DNA-binding sites with different affinities for specific oligonucleotides (ODNs) (K d1=8 nM; K d2∽0.1 mM). The high affinity site became labeled after incubation with affinity probes for DNA-binding sites; like the antibacterial and polyanion-binding sites, this site was shown to be located in the N-terminal domain of LF. Interaction of heparin with the polyanion-binding site inhibits the binding of ODNs to both sites. These data suggest that the DNA-binding sites of LF coincide or overlap with the known polyanion and antimicrobial domains of the protein.Keywords
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