Protoplast fusion within the genus Kluyveromyces van der Walt emend. van der Walt

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Abstract
Fusion experiments, involving protoplasts of selected hybridizing and nonhybridizing Kluyveromyces strains, are reported. The results obtained indicate that prototrophic fusion products were recovered from all 54 crosses involving the protoplasts of hybridizing strains. These products showed varying degrees of stability. Although two different methods of protoplast preparation and protoplast fusion were employed, only 7 out of 32 fusion experiments involving nonhybridizing strains yielded prototrophic fusion products. All these fusion products proved to be unstable after a single passage through a complete medium. reverting directly to the parental strains.

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