Stereochemical studies of enzymatic transglycosylation using Sulfolobus solfataricus

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Abstract
Stereochemistry of β-glycosyl transfer from phenyl β-D-galactoside, lactose, and phenyl β-D-glucoside to various 1,2-, 1,3-, and 1,4-diols, a secondary–tertiary diol, a cyclic diol, and a racemic alcohol has been studied using β-glycosidase activity in a crude preparation from the thermophilic archaebacterium Sulfolobus solfataricus. Good enantioselection for the galactosyl transfer to the secondary hydroxy group of different 1,2-diols has been observed. Good yields in comparison with enzymes from other sources, and results concerning the reaction's regioselectivity, have also been reported.

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