Identification of a tandemly repeated DNA sequence using combined RAPD and FISH in welsh onion(allium Fistulosum)
Open Access
- 1 January 1999
- journal article
- research article
- Published by Taylor & Francis in Korean Journal of Biological Sciences
- Vol. 3 (1) , 69-72
- https://doi.org/10.1080/12265071.1999.9647467
Abstract
A tandemly repeated DNA sequence was identified and characterized by the combined RAPD and FISH data from a total genomic DNA of Welsh onion (Allium fistulosum). A clone containing this repeating sequence was selected and sequenced. This repeating unit of 314 bp inserted into pAf 072 contained 54.1% adenine and thymine residues, and showed the primer sequence used, 5‐GAAACGGGTG‐3’, in both terminals of the sequence. Fluorescence in situ hybridization using this tandemly repeated sequence as a probe indicated that the detected sites were coincident with the major C‐banded constitutive heterochromatin in the terminal regions of both arms of all 16 chromosomesKeywords
This publication has 23 references indexed in Scilit:
- Combined RAPD and RFLP molecular linkage map of asparagusGenome, 1997
- Genome differentiation inAegilops. 2. Physical mapping of 5S and 18S–26S ribosomal RNA gene families in diploid speciesGenome, 1996
- Genome differentiation in Aegilops. 1. Distribution of highly repetitive DNA sequences on chromosomes of diploid speciesGenome, 1996
- Characterization ofHordeum chilensechromosomes by C-banding and in situ hybridization using highly repeated DNA probesGenome, 1995
- Physical mapping of 5S and 18S–25S rDNA and repetitive DNA sequences in Aegilops umbellulataGenome, 1995
- Detection of genetic variation between and within populations of Gliricidia sepium and G. maculata using RAPD markersHeredity, 1992
- Basic local alignment search toolJournal of Molecular Biology, 1990
- Cytological localization of highly repeated DNA sequences, the FokI sequence family and BamHI sequence families, in Vica faba chromosomes.The Japanese Journal of Genetics, 1987
- A molecular description of telomeric heterochromatin in secale speciesCell, 1980
- Heterochromatic patterns in AlliumHeredity, 1976