Viral integration near c-myc in 10-20% of mcf 247-induced AKR lymphomas.

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Abstract
AKR thymomas were induced by injecting retrovirus mink cell focus-forming virus 247 (MCF-247) that had been tagged with a fragment of phage .lambda. in its U3 region. About 10-20% of the 26 primary tumors studied showed both rearranged and germline c-myc bands in Southern blots. The rearranged c-mys genes were cloned from 2 of the tumors and studied by Southern analysis in 2 others. In all 4 cases, rearrangement was due to integration of MCF247 proviruses .apprx. 2 kilobases (kb) 5'' of the 3 c-myc exons and in the opposite transcriptional orientation. Thus, viral integrations were clustered within a 1-kb range in the tumors that revealed c-mys rearrangements. Three of the 4 proviruses appeared to be intact; the 4th had an internal deletion of .apprx. 3 kb.