In vitro induction of vegetative buds in tobacco callus by chelating agents

Publisher Website
Google Scholar
Abstract
Calli obtained from haploid and diploid plants of Nicotiana tabacum were inoculated on nutrient media supplemented with various concentrations of chelating agents: iron salt of ethylenediamine-di-o-hydroxyphenylacetic acid (Fe-EDDHA), 1,3-diamino-2-hydroxypropane-N,N,N′,N′-tetraacetic acid (DHPTA), 1,2-cyclohexanediaminetetraacetic acid (CYDA), citric acid, and tartaric acid. No auxin or cytokinin was added to the medium. Vegetative buds differentiated on the callus obtained from the haploid plants. However, these chelating agents proved ineffective in inducing differentiation on the callus derived from diploid plants. Morphogenetically, Fe-EDDHA and DHPTA proved more potent than CYDA, citric acid, and tartaric acid.

This publication has 0 references indexed in Scilit: