Identification of a wheat germ agglutinin‐sensitive ATPase in yeast nuclei

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Abstract
We have found that wheat germ agglutinin (WGA), a lectin that specifically binds to N‐acetylglucosamine residues inhibits the in vitro transport of plasmid DNA, pJDB219, into yeast nuclei. Histochemical staining of the isolated nuclei with biotinylated WGA and streptavidin‐biotinylated peroxidase complex revealed the presence of WGA‐binding materials around the nuclear pore under an electron microscope. Using WGA‐agarose column chromatography of yeast nuclear extracts, a novel Mg2+‐dependent ATPase was isolated. Its activity was highly sensitive to WGA and stimulated by Nonidet P‐40 or phosphatidylserine. We suggest that the WGA‐sensitive ATPase plays a role in yeast nuclear transport of DNA.