Experimental Analysis of Second-Order Effects on Gravitational Field-Flow Fractionation Retention of Silica Particles

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Abstract
One of the most attractive features of GrFFF is the possibility to perform particle size distribution analysis of supermicron particles dispersions by means of a very simple and inexpensive experimental apparatus, easily obtained by implementing a basic HPLC configuration. Optimization of the method requires the knowledge of the effects (second-order effects) capable of influencing sample retention and, thus, the accuracy of the scaling from the retention times axis to the particle size axis. In the framework of such an optimization, the role played by hydrodynamic effects in modulating retention is here first considered and the experimental verification of the empirical equations describing such effects is presented. The effects exerted on retention by changing different GrFFF systems is also studied by means of such an experimental approach.

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