Cryopreservation of Sheep Red Blood Cells
Open Access
- 1 December 1979
- journal article
- research article
- Published by Springer Nature in Acta Veterinaria Scandinavica
- Vol. 20 (4) , 525-530
- https://doi.org/10.1186/bf03546579
Abstract
The protection of sheep erythrocytes at freezing temperatures was investigated using glycerol, dimethylsulfoxide (DMSO), glucose and four different types of polyvinylpyrrolidone (PVP) as cryoprotective agents. Depending on type (molecular weight) and concentration good protection was obtained with PVP, whereas glycerol, DMSO and glucose were unsatisfactory. Recovery of cells after thawing was most successful when the cells had been frozen at a concentration of 1–2 × 109 cells/ml. No cells tolerated freezing at −20 °G. Best results were obtained when the cells were frozen directly in liquid nitrogen (−196°G).Keywords
This publication has 1 reference indexed in Scilit:
- The cryopreservation of sheep erythrocyte intermediates EA and EAC4 in complement assaysJournal of Immunological Methods, 1974