One‐ and two‐dimensional 1H‐NMR investigations of two 19‐base‐pair analogues of the tet operator
- 1 December 1987
- journal article
- research article
- Published by Wiley in European Journal of Biochemistry
- Vol. 169 (3) , 603-609
- https://doi.org/10.1111/j.1432-1033.1987.tb13651.x
Abstract
Two 19-base-pair oligodeoxynucleotides, analogues of one of the operators which specifically bind the repressor protein in the regulatory part of the transposon Tn10 tetracycline-resistance (tet) determinant, have been studied by 1H-NMR spectroscopy. The analogues contain a mismatch in the central base pair of the double helix (T.cntdot.T or A.cntdot.A). The imino protons have been assigned to the base pairs by one-dimensional NOE measurements, and the thermally induced transition from the duplex to the single strand has been followed. The cytidine amino resonances have been assigned by means of two-dimensional NOE spectroscopy in H2O. Two-dimensional phase-sensitive NOE and magnitude-correlated spectra have been recorded in 2H2O; all nonexchangeable protons, with the exception of some of H5'', H5'''' protons, have been assigned. The NMR data made it possible to carry out a qualitative analysis of the structures of both oligodeoxynucleotides. The general structures close to B-DNA, show irregularities in the mismatch areas.This publication has 38 references indexed in Scilit:
- Z Helix-coil Transition of d(C-Br8G-C-G-C-Br8G) Studied by CD,1H-NMR and IR SpectroscopiesJournal of Biomolecular Structure and Dynamics, 1985
- High Field1H-NMR Analysis of the 1:1 Intercalation Complex of the Antitumor Agent Mitoxantrone and the DNA Duplex [d(CpGpCpG)]2Journal of Biomolecular Structure and Dynamics, 1985
- A nuclear‐Overhauser‐enhancement study of the solution structure of a double‐stranded DNA undecamer comprising a portion of the specific target site for the cyclic‐AMP‐receptor protein in the gal operonEuropean Journal of Biochemistry, 1984
- 1H NMR sudy of the interaction of bacteriophageλ Cro protein with the OR3 operator. Evidence for a change of the conformation of the OR3 operator on bindingNucleic Acids Research, 1984
- Assignment of the non-exchangeable proton resonances of d(C-G-C-G-A-A-T-T-C-G-C-G) using two-dimensional nuclear magnetic resonance methodsJournal of Molecular Biology, 1983
- Sequential resonance assignments in DNA proton NMR spectra by two-dimensional NOE spectroscopyJournal of the American Chemical Society, 1983
- Kinetics for exchange of the imino protons of the d(C-G-C-G-A-A-T-T-C-G-C-G) double helix in complexes with the antibiotics netropsin and/or actinomycinBiochemistry, 1983
- Proton nuclear magnetic resonance study of a self-complementary decadeoxyribonucleotide, C-C-A-A-G-C-T-T-G-GBiochemistry, 1982
- Use of two-dimensional NMR in the study of a double-stranded DNA decamerJournal of the American Chemical Society, 1982
- Premelting and melting transitions in the d(CGCGAATTCGCG) self-complementary duplex in solutionBiochemistry, 1982