Characterization of a factor inducing differentiation of mouse myeloid leukemic cells purified from conditioned medium of mouse Ehrlich ascites tumor cells

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Abstract
A factor inducing differentiation of mouse myeloid leukemic cells (M1) into macrophages was purified to apparent homogeneity from 168 1 of CM of Ehrlich ascites tumor cells. The purified factor was halt-maximally active at 2 × 10−11 M. The factor was analyzed by radioiodination, SDS-polyacrylamide gel electrophoresis and autoradiography. Its Mr was 40000–50000. On reduction, the factor lost activity, but showed no subunit structure. Treatment of the factor with endo-β-N-acetylglucosaminidase F, but not endo-β-N-acetylglucosaminidase H, gave rise to a molecule of Mr 20000–28000. The activity of the factor from Ehrlich cells was completely neutralized by antiserum to the factor of Mr 50000–70000 from mouse fibroblast L929 cells