TWO SELECTIVE ENRICHMENT MEDIA FOR THE ISOLATION OF SALMONELLA FROM MECHANICALLY DEBONED POULTRY MEAT

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Abstract
Two selective enrichment media were developed for the isolation of salmonellae from foods. The first, experimental broth 1, contained magnesium chloride and sodium cholate as the selective ingredients. The second, experimental broth 2, contained 8 mg/1 sodium sulfadiazine in addition to magnesium chloride and sodium cholate. The two broths were evaluated against conventional selective enrichment media in the isolation of salmonellae from naturally contaminated mechanically deboned poultry meat. Of 100 such samples, 22 were positive for Salmonella after enrichment in selenite cystine broth, 12 were positive after enrichment in tetrathionate broth, 50 were positive after enrichment in experimental broth 1, and 60 samples were positive after enrichment in experimental broth 2. The inhibition of interfering Gram negative organisms was significantly greater in the two experimental broths than in the conventional selective enrichment broths.