Immobilization of Escherichia coli expressing the lux genes of Xenorhabdus luminescens
- 1 October 1994
- journal article
- research article
- Published by American Society for Microbiology in Applied and Environmental Microbiology
- Vol. 60 (10) , 3862-3863
- https://doi.org/10.1128/aem.60.10.3862-3863.1994
Abstract
The luxCDABE operon of Xenorhabdus luminescens was cloned into pUC18 to make pLITE27. Expression of the lux genes from the lac promoter resulted in strong constitutive light emission by Escherichia coli DH5 carrying the recombinant lux plasmid, pLITE27. When strain DH5(pLITE27) was immobilized with sodium alginate-CaCl2, the embedded cells retained their luminescence up to 2 weeks under appropriate storage conditions.Keywords
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