Protection against fatalPseudomonas aeruginosa sepsis by immunization with smooth and rough lipopolysaccharides

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Abstract
The protective capacity of various native and mutant lipopolysaccharide antigens against fatalPseudomonas aeruginosa burn wound sepsis was evaluated. Immunization with O-polysaccharide-deficient lipopolysaccharides derived fromEscherichia coli J5 orSalmonella typhi Ty 21a afforded substantial protection against only one of fivePseudomonas aeruginosa challenge strains of various serotypes. Immunization with both lipopolysaccharide antigens evoked antibody of the immunoglobulin G class which recognized lipopolysaccharide isolated from the challenge strain against which protection was noted. This was not seen for the remaining four challenge strains. Attempts to demonstrate cross-serotype protection using O-antigen-deficient and core-deficientPseudomonas aeruginosa lipopolysaccharide antigens was, for the most part, unsuccessful. In contrast, high levels of protection against all six serotypes of challenge strains were seen following immunization with homologous lipopolysaccharide.