Evaluation of the Oxi/Ferm tube system for identification of nonfermentative Gram-negative bacilli

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Abstract
The Oxi/Ferm tube system designed for identification of oxidative-fermentative gram-negative bacteria was evaluated for identification of nonfermentative gram-negative bacilli by comparing it with the conventional system. The 9 biochemical reactions used as the initial tests in the Oxi/Ferm tube system showed a close agreement with the same conventional reactions. The system was only 41% accurate to species or biotype for complete identification of 239 isolates which were well distributed in 48 species and biotypes and included organisms not listed in the Oxi/Ferm tube identification list. The system correctly identified 56% of the test organisms when the degree of identification was based on the manufacturer''s guidance list. All isolates of Acinetobacter calcoaceticus, Bordetella bronchiseptica, Pseudomonas aeruginosa, P. diminuta and group IIf were completely identified by the system.