Primary structure of a major sialyl‐saccharide alditol from human amniotic mucins expressing the tumor‐associated sialyl‐X antigenic determinant

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Abstract
A major sialyl‐saccharide alditol isolated from mucins of human amniotic fluid was purified by high‐performance liquid chromatography followed by high‐performance thin‐layer chromatography of the methylated derivative and subjected to structural analyses which comprised fast atom bombardment and electron impact‐mass spectrometry, methylation analysis, exoglycosidase digestion and CrO3 oxidation. The structure of a disialylated pentasaccharide characterized by a sialyl‐X antigenic determinant was established:

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