Conformationally Correct Expression of Membrane-AnchoredToxoplasma gondiiSAG1 in the Primitive ProtozoanGiardia duodenalis
- 1 February 2002
- journal article
- Published by American Society for Microbiology in Infection and Immunity
- Vol. 70 (2) , 1014-6
- https://doi.org/10.1128/iai.70.2.1014-1016.2002
Abstract
To explore the possibility of expressing membrane-anchored exodomains of heterologous surface antigens inGiardia, a chimeric construct containing theToxoplasma gondii SAG1gene was made. TheGiardiasystem is shown here to provide a means of generating correctly folded chimeric surface proteins in a native and unmodified form.Keywords
This publication has 14 references indexed in Scilit:
- Characterization of Toxoplasma gondii surface antigen 1 (SAG1) secreted from Pichia pastoris : evidence of hyper O‐glycosylationBiotechnology and Applied Biochemistry, 2001
- Glycosylation and palmitoylation are common modifications of Giardia variant surface proteinsMolecular and Biochemical Parasitology, 2000
- Stage-Specific Expression and Targeting of Cyst Wall Protein–Green Fluorescent Protein Chimeras inGiardiaMolecular Biology of the Cell, 2000
- Baculovirus-directed expression and secretion of a truncated version of Toxoplasma SAG1Molecular and Biochemical Parasitology, 1999
- Analysis of Toxoplasma gondii stably transfected with a transmembrane variant of its major surface protein, SAG1Journal of Cell Science, 1998
- The variant-specific surface protein of Giardia, VSP4A1, is a glycosylated and palmitoylated proteinBiochemical Journal, 1997
- Giardia lamblia: Post-translational Processing and Status of Exposed Cysteine Residues in TSA 417, a Variable Surface AntigenExperimental Parasitology, 1993
- Variant surface antigens of Giardia lamblia are associated with the presence of a thick cell coat: thin section and label fracture immunocytochemistry surveyInfection and Immunity, 1991
- Antigenic variation of Giardia lamblia in experimental human infections.The Journal of Immunology, 1990
- Isolation and expression of the gene for a major surface protein of Giardia lamblia.Proceedings of the National Academy of Sciences, 1990