Breakage of single-stranded DNA by rat liver nicking-closing enzyme with the formation of a DNA-enzyme complex
Open Access
- 1 January 1980
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 8 (24) , 6129-6142
- https://doi.org/10.1093/nar/8.24.6129
Abstract
The DNA nicking-closing enzyme (type I topoisomerase) from rat liver nuclei breaks single-stranded DNA. The broken strand contains a 5′-hydroxyl and tightly bound protein. The stability of this protein-DNA complex to high salt, alkali and detergent suggests a covalent linkage between the DNA and the enzyme. The observed breakage of single-stranded DNA occurs at neutral pH prior to treatment with alkali or detergent, indicating that the breakage may be the result of an interrupted nicking and closing cycle. The resulting covalent complex could represent a reaction intermediate in the overall nicking-closing reaction.Keywords
This publication has 20 references indexed in Scilit:
- Strand breakage by the DNA untwisting enzyme results in covalent attachment of the enzyme to DNA.Proceedings of the National Academy of Sciences, 1977
- ϕX174 cistron A protein is a multifunctional enzyme in DNA replicationProceedings of the National Academy of Sciences, 1977
- Purification and characterization of the DNA untwisting enzyme from rat liverBiochemistry, 1976
- Evidence for an intermediate with a single-strand break in the reaction catalyzed by the DNA untwisting enzyme.Proceedings of the National Academy of Sciences, 1976
- Knotted single-stranded DNA rings: A novel topological isomer of circular single-stranded DNA formed by treatment with Escherichia coli ω proteinJournal of Molecular Biology, 1976
- Assay of RNA-linked nascent DNA pieces with polynucleotide kinaseBiochemical and Biophysical Research Communications, 1975
- Priming of superhelical SV40 DNA by Escherichia coli RNA polymerase for in vitro DNA synthesisBiochemistry, 1975
- Characterization of the replicative intermediates of polyoma virusVirology, 1974
- An Activity from Mammalian Cells That Untwists Superhelical DNA—A Possible Swivel For DNA ReplicationProceedings of the National Academy of Sciences, 1972
- Interaction between DNA and an Escherichia coli protein ωJournal of Molecular Biology, 1971