Use of Micellar Mobile Phases and an HPLC Column Switching System for Direct Injection Determination of Urinary Free Cortisol
- 1 August 1993
- journal article
- research article
- Published by Taylor & Francis in Journal of Liquid Chromatography
- Vol. 16 (12) , 2583-2594
- https://doi.org/10.1080/10826079308019594
Abstract
A direct injection method for the determination of urinary free Cortisol by an HPLC column switching system is presented. The first chromatographic column, which provides for sample preconcentration and clean-up, employs a micellar mobile phase. The second column, coupled on -line to the first, utilizes reversed-phase condition for analysis. UV detection is employed at 254 nm. Detection limit for urinary free Cortisol is 2.4 ng when 2 ml urine sample is injected. Nine samples were analyzed. The results were in a reasonable range. This method can be used successfully in the diagnosis of patients with hypercortisolism and Cushing's syndrome.Keywords
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