Quantitative Analysis of Peptides by Matrix-Assisted Laser Desorption/Ionization Time-Of-Flight Mass Spectrometry
- 1 August 1993
- journal article
- Published by SAGE Publications in Applied Spectroscopy
- Vol. 47 (8) , 1091-1092
- https://doi.org/10.1366/0003702934067847
Abstract
Quantitative analysis of peptides using internal standards was carried out with the use of matrix-assisted laser desorption/ionization (MALDI) on a time-of-flight mass spectrometer in a ferulic acid matrix. Spectra were collected and averaged over several areas of the sample surface at constant laser energy, and a defocused beam was used to improve reproducibility. A calibration curve was plotted, showing a correlation coefficient of 0.992 and a relative standard deviation of 1.83%. The accuracy and precision of this technique were sensitive to the crystal structure of the matrix and saturation effects of the instrument's detector.Keywords
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