The expression of cecropin peptide in transgenic tobacco does not confer resistance to Pseudomonas syringae pv tabaci
- 1 February 1994
- journal article
- Published by Springer Nature in Plant Cell Reports
- Vol. 13 (5)
- https://doi.org/10.1007/bf00233324
Abstract
We used in vitro growth inhibition assays to demonstrate that synthetic cecropin protein has potent activity against a range of plant pathogenic bacteria. We then prepared transgenic tobacco plants which express cecropin mRNA and protein. We have used Pseudomonas syringae pv tabaci infection of these transgenic tobacco as a model system to evaluate whether the plants which express cecropin protein also have increased tolerance to infection. We found no dramatic difference in disease response between plants which are expressing cecropin protein and control plants which were derived from the transformation with a binary vector which did not carry the gene encoding cecropin protein.Keywords
This publication has 13 references indexed in Scilit:
- Protein secretion in plant cells can occur via a default pathway.Plant Cell, 1990
- Channel-forming properties of cecropins and related model compounds incorporated into planar lipid membranes.Proceedings of the National Academy of Sciences, 1988
- Cell-Free Immunity in InsectsAnnual Review of Microbiology, 1987
- Simple binary vectors for DNA transfer to plant cellsPlant Molecular Biology, 1985
- Molecular cloning, cDNA sequencing, and chemical synthesis of cecropin B from Hyalophora cecropia.Proceedings of the National Academy of Sciences, 1985
- N-Terminal analogs of cecropin A: synthesis, antibacterial activity, and conformational propertiesBiochemistry, 1985
- Immunogenic structure of the influenza virus hemagglutininCell, 1982
- Sequence and specificity of two antibacterial proteins involved in insect immunityNature, 1981
- [8] Use of glutaraldehyde as a coupling agent for proteins and peptidesPublished by Elsevier ,1980
- A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye BindingAnalytical Biochemistry, 1976