Characterization of the Chemoenzymatic Synthesis of N‐Acetyl‐d‐neuraminic Acid (Neu5Ac)
- 1 January 1996
- journal article
- Published by Wiley in Biotechnology Progress
- Vol. 12 (6) , 758-763
- https://doi.org/10.1021/bp9600647
Abstract
The characterization of the chemoenzymatic synthesis of N‐acetyl‐d‐neuraminic acid using a base‐catalyzed epimerization and Neu5Ac aldolase‐catalyzed condensation is presented. The characterization has been used to exemplify a structured approach to biotransformation process design. In addition, the option of integration of the epimerization with the biotransformation (at alkaline pH) was considered. Process operating windows have been generated as a result of this approach, defining possible operating regions for subsequent scale‐up.Keywords
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