Role of C4-Binding Protein in the Defective Lysis of Sensitized Sheep Erythrocytes by Mouse Complement

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Abstract
Passage of mouse EDTA-plasma over an anti-C4-binding protein (anti-C4-bp) affinity column reduced the C4-bp level by an average of 34% and increased the classical C pathway activity as measured with sensitized normal and desialylated sheep erythrocytes, but not versus sensitized rabbit erythrocytes. This suggests that C-regulation by C4-bp is independent of membrane sialic acid and provides evidence for the presence of a hitherto unknown preferential site for mouse C4-bp on sheep erythrocytes. The absence of both this site and sialic acid on their membranes makes sensitized rabbit erythrocytes useful target cells for assay of mouse classical C pathway activity.

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