WAVELENGTH DEPENDENCE FOR AMT CROSSLINKING OF pBR322 DNA
- 1 August 1984
- journal article
- research article
- Published by Wiley in Photochemistry and Photobiology
- Vol. 40 (2) , 215-219
- https://doi.org/10.1111/j.1751-1097.1984.tb04578.x
Abstract
The wavelength dependence for 4′aminomethyl‐4,5′,8‐trimethylpsoralen crosslinking of a linearized plasmid DNA (pBR322) by narrow band UV‐A light (298–382 nm) has been determined. Maximal levels of crosslinking occurred with light in the 322–346 nm range. Crosslinks were shown to be photoreversible by shorter wavelength photons (298 and 310 nm). The correlation between the wavelength dependence for crosslink formation and the optimal wavelength for most psoralen action spectra further supports the notion that crosslinks are the major lesion responsible for the effectiveness of psoralen plus ultraviolet A therapies.This publication has 18 references indexed in Scilit:
- A fluorescent complex between ethidium bromide and nucleic acids: Physical—Chemical characterizationPublished by Elsevier ,2004
- Induction and repair of psoralen cross-links in DNA of normal human and xeroderma pigmentosum fibroblastsMutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 1982
- AN INSTRUMENT FOR ACTION SPECTRUM STUDIES IN DERMATOLOGYPhotochemistry and Photobiology, 1982
- Psoralen Photochemistry and Nucleic Acid StructureJournal of Investigative Dermatology, 1981
- Isolation and characterization of pyrimidine-psoralen photoadducts from DNAJournal of the American Chemical Society, 1981
- SENSITIVITY OF DNA REPAIR‐DEFICIENT STRAINS OF ESCHERICHIA COLI K‐12 TO VARIOUS FUROCOUMARINS AND NEAR‐ULTRAVIOLET RADIATIONPhotochemistry and Photobiology, 1981
- PHOTOCHEMISTRY AND PHOTOBIOLOGY OF PSORALENSPhotochemistry and Photobiology, 1979
- KINETIC ANALYSIS OF THE PHOTOREACTION (365 nm) BETWEEN PSORALEN AND DNAPhotochemistry and Photobiology, 1979
- ACTION SPECTRUM FOR THE PHOTO‐INACTIVATION OF LAMBDA AND T4 BACTERIOPHAGES SENSITIZED WITH 8‐METHOXYPSORALENPhotochemistry and Photobiology, 1977
- Excited states of skin-sensitizing coumarins and psoralens. Spectroscopic studiesJournal of the American Chemical Society, 1973