Determination of the Proportion of Cholesteryl Ester in Plasma

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Abstract
In normal human plasma the proportion of cholesterol present as cholesteryl ester is very constant, but in certain clinical conditions, such as liver disease, a reduction may occur. TLC was used to separate free cholesterol and cholesteryl ester fractions followed by hydrolysis of both under identical conditions without prior elution from the silica gel. The non-saponifiable material was then extracted into hexane, and aliquots were taken for colorimetric estimation.

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