Effect of Light Quality on Chloroplast Replication in Spinach

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Abstract
Chloroplast growth and replication have been studied in spinach leaf dises cultured on sterile nutrient agar under lights of different spectral qualities. Over the intensity range 0.1-5 mW cm−2 both red (632 nm) and blue (488 nm) laser light stimulate chloroplast division to the same extent as white light. By contrast low intensities (0.22–0.65 m Wcm−2 of both white or green (525 nm) light are ineffective for chloroplast replication but permit normal chlorophyll synthesis and greater than normal chloroplast growth. The large plastids of dises grown in green light divide when exposed to high-intensity white light.

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