Temporal and spatial regulation of the symbiotic genes of Rhizobium meliloti in planta revealed by transposon Tn5-gusA.
Open Access
- 1 March 1990
- journal article
- research article
- Published by Cold Spring Harbor Laboratory in Genes & Development
- Vol. 4 (3) , 344-356
- https://doi.org/10.1101/gad.4.3.344
Abstract
Tn5-gusA promoter/probe transposons have been constructed that fuse the Escherichia coli gusA reporter gene transcriptionally or translationally with a target promoter. These have been used to monitor expression of Rhizobium meliloti symbiotic genes within alfalfa nodules. Fusions in all 11 nod genes studied show the same pattern of expression: first on the root surface, then throughout the developing nodule, then mainly in the nodule meristem, falling off progressively through the central region, and then disappearing. In contrast, fusions in all five nif genes studied, all four fix genes, and syrM show a second, different pattern: expression beginning later, first throughout the nodule except for the meristem, strongest just behind the meristem, and falling off progressively through the central region. Novel features revealed by these studies include nod expression in the meristem, regulated in planta expression of control genes nodD1 and nodD3, disappearance of nod expression late in organogenesis, and properties of syrM.Keywords
This publication has 64 references indexed in Scilit:
- The Rhizobium meliloti host range nodQ gene encodes a protein which shares homology with translation elongation and initiation factors.Molecular Microbiology, 1989
- A novel exopolysaccharide can function in place of the Calcofluor-binding exopolysaccharide in nodulation of alfalfa by Rhizobium melilotiCell, 1989
- ThenodHandnodQHost Range Genes ofRhizobium melilotiBehave as Avirulence Genes inR. leguminosarumbv.viciaeand Determine Changes in the Production of Plant-Specific Extracellular SignalsMolecular Plant-Microbe Interactions®, 1989
- The Relationship between Nodulin Gene Expression and theRhizobium nodGenes inVicia sativaRoot Nodule DevelopmentMolecular Plant-Microbe Interactions®, 1989
- Rhizobium melilotiGenes for Exopolysaccharide Synthesis and Nodule Infection Located on Megaplasmid 2 Are Actively Transcribed during SymbiosisMolecular Plant-Microbe Interactions®, 1988
- Rhizobium nod genes are involved in inducing an early nodulin geneNature, 1986
- The nodI gene product of Rhizobium leguminosarum is closely related to ATP-binding bacterial transport proteins; nucleotide sequence analysis of the nodI and nodJ genesGene, 1986
- Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mpl8 and pUC19 vectorsGene, 1985
- DNA sequences at the ends of transposon Tn5 required for transpositionNature, 1983
- Leghemoglobin biosynthesis in soybean root nodules. Characterization of the nascent and released peptides and the relative rate of synthesis of the major leghemoglobinsBiochemistry, 1979